| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Pelletier, Ava E (10)
|
NCGFSC |
42.77 |
42.48 |
-0.29 |
| 2 |
Milligan, Sarah M (10)
|
NCRMY |
46.66 |
43.57 |
-3.09 |
| 3 |
Hyson, Isla G (10)
|
NCNSEA |
49.65 |
44.77 |
-4.88 |
| 4 |
Milne, Eleanor G (10)
|
NCGFSC |
44.71 |
45.79 |
1.08 |
| 5 |
Norris, Stella E (9)
|
NCGFSC |
48.24 |
46.49 |
-1.75 |
| 6 |
Norris, Scarlett J (9)
|
NCGFSC |
45.64 |
46.69 |
1.05 |
| 7 |
Mann, Emily L (10)
|
NCRMY |
|
48.50 |
- |
| 8 |
Lovsin, Colette S (9)
|
NCNEAL |
51.32 |
49.72 |
-1.60 |
| 9 |
Tranel, Nyah L (10)
|
NCNEAL |
51.09 |
50.08 |
-1.01 |
| 10 |
Stocks, Kinsley L (9)
|
NCAQUA |
55.25 |
52.61 |
-2.64 |
| 11 |
Moore, Maison E (10)
|
NCTRY |
59.86 |
56.09 |
-3.77 |
| 12 |
Rollins, Emery R (10)
|
NCWBST |
59.68 |
57.12 |
-2.56 |
| 13 |
Kibbe, Elizabeth E (9)
|
NCRMY |
1:03.84 |
57.48 |
-6.36 |
| 14 |
Nehus, Olivia E (9)
|
NCAQUA |
|
1:06.84 |
- |
| 15 |
Burckhardt, Adyson C (9)
|
NCHCAC |
1:04.79 |
1:08.26 |
3.47 |
|
Greene, Reagan C (10)
|
NCAQUA |
|
DQ |
- |
|
Havenstein, Lindsey T (10)
|
NCNEAL |
49.81 |
NS |
- |
|
Ciancanelli, Isabella R (10)
|
NCGFSC |
43.07 |
NS |
- |
|
Ivins, Grace M (9)
|
NCNSEA |
1:01.41 |
NS |
- |